Ikhithi isetshenziselwa ukuthola i-in vitro qualitative gene ye-ORF1ab/N/S yenoveli coronavirus 2019-nCoV ezifanekisweni zokuphefumula ezihlanganisa i-oropharyngeal swabs, i-nasopharyngeal swab, isikhwehlela kanye nokwenza ukuthayipha kwe-mutation ku-HV69- 70 del kanye kanye.Njengoba kubikiwe kusizindalwazi se-GISAID, ukulandelana okuhlukile kwe-Omicron okungaphezu kuka-95% okubikiwe kufaka phakathi ukususwa ku-HV69-70, okungabangela ukwehluleka kwethagethi yofuzo lwe-S (SGTF) ekuhlolweni kwe-PCR.I-SGTF ingasetshenziswa njengomaka wommeleli ukuze uhlolele i-Omicron.
Amasethi e-Primer kanye ne-FAM enelebuli yophenyo yakhelwe ukutholwa okuthile kofuzo lwelebhu ye-ORF ka-2019-nCoV, i-VIC enelebuli ye-N gene ka-2019-nCoV, i-ROX enelebuli ye-probe ye-S gene HV69-70 del mutation of 2019-nCoV.Ufuzo lomuntu lwe-RNase P olukhishwe kanyekanye nesampula yokuhlola lunikeza ukulawula kwangaphakathi ukuze kuqinisekiswe inqubo yokukhipha i-nucleic kanye nobuqotho be-reagent.Uphenyo oluqondiswe kufuzo lomuntu lwe-RNase P lubhalwe ukuthi CY5.
Isistimu ye-PCR Yesikhathi Sangempela: I-Aehealth Diagenex AL, ABI 7500, ViiATM 7, Quant Studio 7 flex.Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-GeneTM6000/0.I-Bio-Rad CEX96 TouchTM SLAN-96S.SLAN-96P, MA 6000;