Le kit isetshenziselwa ukutholwa kwe-in vitro qualitative nucleic acid ye-Monkeypox Virus kanye ne-central/west African clade ku-Human serum, amasampula e-lesion exudate kanye nezibonelo zotwayi.Amasethi e-primer kanye nama-probe anelebuli ye-FAM aklanyelwe ukutholwa kwe-Monkeypox Virus, i-ROX enelebuli yophenyo lwe-clade emaphakathi ne-Afrika, i-CY5 enelebula i-probe ye-West African clade.Ufuzo lomuntu lwe-RNase P olukhishwe kanyekanye nesampula yokuhlola lunikeza ukulawula kwangaphakathi ukuze kuqinisekiswe inqubo yokukhipha i-nucleic kanye nobuqotho be-reagent.Uphenyo oluqondiswe kufuzo lomuntu lwe-RNase P lubhalwe ukuthi VIC.
| Izingxenye | 48 Izivivinyo/ikhithi | 96 Izivivinyo/ikhithi |
| Ibhafa yokusabela ye-PCR | 672 μL × 1 ishubhu | 672 μL × 2 ithubhu |
| Ingxube ye-enzyme ye-PCR | 50 μL × 1 ishubhu | 100 μL × 1 ishubhu |
| Ukulawula okuhle | 100 μL × 1 ishubhu | 200 μL × 1 ishubhu |
| Ukulawula okungalungile | 100 μL × 1 ishubhu | 200 μL × 1 ishubhu |
1. Ukuzwela: amakhophi angu-200/mL.
2. Ukucaciswa: Akukho ukusabela okuphambene ne-Enterovirus(EV), Igciwane lesimungumungwane(MV), Rubella virus(RV), Varicella-zoster virus(VZV), Dengue virus(DenV), Human Parvovirus B19(HPVB19), Epstein-barr virus (EBv), i-herpes virus 6(HHV-6)
3. Ukunemba: I-CV ≤ 5%.
Isistimu ye-PCR Yesikhathi Sangempela:Diagenex AL, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P/3005P, Rotor-GeneTM 6000/Q, Bio-Rad CFX96 TouchTM/iQTM ，Hongshi SLAN -96S/96P,AGS8830, AGS4800