Isizinda se-Spike glycoprotein receptor-binding (RBD) se-SARS-CoV-2 silamula ukubophezela kwezinhlayiyana zegciwane kumamukeli we-angiotensin-converting enzyme 2 (ACE2) ebusweni bamaseli omuntu.Ngakho-ke, ukusebenzisana kwe-Spike-ACE2 kuyisici esibalulekile sokunquma ukutheleleka ngegciwane.Iqembu elisha le-phylogenetic le-SARS-CoV-2 (uhlu lozalo B.1.1.7), elifaka esikhundleni se-amino acid ku-Spike RBD (ukuguqulwa kwe-N501Y).
Ngokocwaningo olusha oluvela eMnyangweni Wezempilo wase-UK, olususelwe kudatha yokulandelana kofuzo lonke, i-epidemiology kanye nokumodela, ukusuleleka kohlobo oluhlukile lwe-SARS-CoV-2 cishe kuphakeme ngama-40-70% kunomunye usosayensi otholakalayo wokuguqula. .Ukusabela okusheshayo nokutholwa kungenye yezinyathelo ezibalulekile zokuvimbela nokulawula.
Ikhithi isetshenziselwa i-in vitro qualitative ukuthola uhlobo lwe-ORF1ab/N/S yenoveli coronavirus 2019-nCoV ezifanekisweni zokuphefumula, nokwenza ukuthayipha kokuguqula ku-N501Y ngesikhathi esisodwa.
Ikhithi isebenzisa indlela ye-ARMS, ithatha isakhi sofuzo sika-S se-novel coronavirus (2019-nCoV) njengendawo okuhloswe ngayo ukuthayipha ngokushintshashintsha, idizayine iziqalo ezithile kanye nophenyo lwe-N501Y.
| Imikhiqizo | Imininingwane | Izinga lokushisa lesitolo | Incazelo |
| Ikhithi ye-PCR yesikhathi sangempela yeNoveliICoronavirus 2019-nCoV kanyeI-Novel Coronavirus 2019-nCoVI-Gene Mutation (N501Y) | 48T | -20 ℃ | Abahlaziyi: Wild-uhlobo lwe-N501Y,Uhlobo oluguquguqukayo lwe-N501Y, ORF1ab,I-N gene nokulawula kwangaphakathi |
Ukuvumelana kwesifanekiso
- I-Oropharyngeal Swabs
- I-Nasopharyngeal Swab
- I-Bronchoalveolar Lavage Fluid
- Isikhwehlela
Izingxenye Zekhithi
- Ibhafa yokusabela ye-RT-PCR
- Ingxube ye-enzyme ye-RT-PCR
- Ukulawula okuhle kohlobo lwasendle
- Ukulawula okuhle okushintshiwe
- Ukulawula okungalungile
Uhlobo lokuguqulwa kwe-N501Y
