I-Rapid COVID-19 Antigen Test iyi-colloidal gold immunochromatography ehloselwe ukutholwa kwekhwalithi yama-nucleocapsid antigens avela ku-COVID-19 emakhaleni omuntu, ama-swabs omphimbo noma amathe avela kubantu okusolwa ukuthi bane-COVID-19 ngumhlinzeki wabo wezempilo.Ama-coronavirus anoveli awohlobo lwe-β.I-COVID-19 yisifo esithathelwanayo sokuphefumula esiyingozi.Ngokuvamile abantu bayathinteka.Njengamanje, iziguli ezingenwe yi-coronavirus yenoveli ziwumthombo oyinhloko wokutheleleka;abantu abane-asymptomatic abangenwe yileli gciwane bangaba umthombo othathelwanayo.Ngokusekelwe ophenyweni lwamanje lwe-epidemiological, isikhathi sokufukamela siyizinsuku ezi-1 kuya kweziyi-14, izikhathi eziningi izinsuku ezi-3 kuya kweziyi-7.Izimpawu eziyinhloko zihlanganisa umkhuhlane, ukukhathala nokukhwehlela okomile.Ukucinana kwamakhala, ikhala eligijimayo, umphimbo obuhlungu, i-myalgia kanye nesifo sohudo kutholakala ezimweni ezimbalwa.Imiphumela ingeyokuhlonza i-COVID-19 nucleocapsid antigen.I-antigen ngokuvamile itholakala kumasampula aphezulu okuphefumula noma amasampula aphansi okuphefumula phakathi nesigaba esibucayi sokutheleleka.Imiphumela emihle ibonisa ukuba khona kwama-antigen amagciwane, kodwa ukuhlobana komtholampilo nomlando wesiguli kanye nolunye ulwazi lokuxilonga kuyadingeka ukuze kunqunywe isimo sokutheleleka.Imiphumela emihle ayikuvimbeli ukutheleleka ngebhaktheriya noma ukutheleleka okuhlangene namanye amagciwane.I-antigen etholakele ingase ingabi imbangela eqondile yesifo.Imiphumela engemihle ayikukhiphi ukutheleleka nge-COVID-19 futhi akufanele isetshenziswe njengesisekelo sodwa sokwelashwa noma izinqumo zokuphatha isiguli, okuhlanganisa nezinqumo zokulawula ukutheleleka.Imiphumela engemihle kufanele icatshangelwe kumongo wokuchayeka kwakamuva kwesiguli, umlando kanye nokuba khona kwezimpawu zomtholampilo nezimpawu ezihambisana ne-COVID-19 futhi kuqinisekiswe ngokuhlolwa kwe-molecular, uma kudingekile ukuze kuphathwe isiguli.
Lesi sici sisekelwe ku-colloidal gold immunochromatography assay.Phakathi nokuhlolwa, okukhishwe kwesifanekiso kusetshenziswa Emakhadini Okuhlola.Uma kukhona i-COVID-19 antigen esikhiqizweni, i-antigen izobophezela ku-COVID-19 monoclonal antibody.Ngesikhathi sokugeleza kwe-lateral, inkimbinkimbi izohamba ngolwelwesi lwe-nitrocellulose luye ekupheleni kwephepha elimuncayo.Lapho wedlula umugqa wokuhlola (umugqa T, ohlanganiswe nenye i-COVID-19 monoclonal antibody) inkimbinkimbi ithwetshulwa amasosha omzimba e-COVID-19 kulayini wokuhlola ibonisa umugqa obomvu;lapho kudlula umugqa C, imbuzi emelene nonogwaja ebhalwe ngegolide ye-colloidal IgG ibanjwa ngomugqa wokulawula (umugqa C, ombozwe ngonogwaja IgG) ukhombisa umugqa obomvu.
Izingxenye ezilandelayo zifakiwe kukhithi yokuhlola ye-Rapid COVID-19 Antigen.
Izinto Ezinikeziwe:
| Uhlobo lwesampula | Izinto zokwakha |
|
Amathe(kuphela) |
|
Izinto Ezidingekayo Kodwa Ezingakahlinzeki:
1. Isikhathi
2. I-tube rack yama-specimens
1. Gcina umkhiqizo ku-2-30℃, impilo yeshalofu yizinyanga ezingama-24 ngokuzenzakalelayo.
2. Ikhasethi lokuhlola kufanele lisetshenziswe ngemva nje kokuvula isikhwama.
3. Ama-reagents namadivayisi kufanele kube kuzinga lokushisa elilingana negumbi(15-30℃) uma kusetshenziselwa ukuhlola.
Iqoqo Lesampula LeSwab Somphimbo:
Vumela ikhanda lesiguli litsheke kancane, uvule umlomo, futhi wenze imisindo ethi "ah", eveza amathoni e-pharyngeal ezinhlangothini zombili.Bamba i-swab bese usula amathoni e-pharyngeal ezinhlangothini zombili zesiguli ngamandla aphakathi emuva naphambili okungenani izikhathi ezingu-3.
Ukuqoqwa Kwesampula Samathe nge-Swab:
Ukuqoqwa Kwesampula Ye-saliva ngedivayisi yokuqoqwa kwamathe:
Isampula Ezokuthutha Nesitoreji:
Amasampula kufanele ahlolwe ngokushesha ngangokunokwenzeka ngemva kokuqoqwa.Ama-swabs noma isifanekiso samathe singagcinwa ku-Extraction Solution kuze kufike emahoreni angama-24 ekamelweni lokushisa noma ku-2° ukuya ku-8°C.Ungaqandi.
1. Ukuhlolwa kufanele kusetshenziswe ekamelweni lokushisa (15-30 ° C).
2. Engeza izibonelo.
Isifanekiso samathe (kusuka kudivayisi yokuqoqa amathe):
Okuhle
Kukhona umbala kulayini C, futhi umugqa onombala uvele umugqa T olula kunomugqa C, noma lapho
Okubi
Kukhona umbala kulayini C, futhi umugqa onombala uvele umugqa T omnyama noma olingana no
Akuvumelekile
Awukho umbala kulayini C, njengoba kukhonjisiwe ezithombeni ezilandelayo.Ukuhlola akuvumelekile noma kuyiphutha
Negative(-): Imiphumela engemihle iyaqagela.Imiphumela yokuhlolwa engemihle ayikuvimbeli ukutheleleka futhi akufanele isetshenziswe njengesisekelo sodwa sokwelashwa noma ezinye izinqumo zokuphatha isiguli, okuhlanganisa izinqumo zokulawula ukutheleleka, ikakhulukazi uma kukhona izimpawu zomtholampilo nezimpawu ezihambisana ne-COVID-19, noma kulabo abake bagula. ukuthintana negciwane.Kunconywa ukuthi le miphumela izoqinisekiswa ngendlela yokuhlola i-molecular, uma kunesidingo, yokulawulwa kokuphathwa kwesiguli.
Okuhle(+): Kuhle ngokuba khona kwe-antigen yakwa-SARS-CoV-2.Imiphumela emihle ibonisa ukuba khona kwama-antigen egciwane, kodwa ukuhlobana komtholampilo nomlando wesiguli kanye nolunye ulwazi lokuxilonga kuyadingeka ukuze kunqunywe isimo sokutheleleka.Imiphumela emihle ayikukhiphi ukutheleleka ngebhaktheriya noma ukuhlangana namanye amagciwane.I-antigen etholakele ingase ingabi imbangela eqondile yesifo.
1.Ukusebenza komtholampilo kwahlolwa ngamasampula aqandisiwe, futhi ukusebenza kokuhlolwa kungase kuhluke ngamasampula amasha.
2.Abasebenzisi kufanele bahlole izifanekiso ngokushesha ngangokunokwenzeka ngemva kokuqoqwa kwesifanekiso.
3. Imiphumela yokuhlolwa emihle ayikukhiphi ukutheleleka okuhlangene namanye amagciwane.
4. Imiphumela yokuhlolwa kwe-COVID-19 antigen kufanele ihlotshaniswe nomlando womtholampilo, idatha ye-epidemiological, kanye nolunye ulwazi olutholakala kudokotela ohlola isiguli.
5.Umphumela wokuhlola ongelona iqiniso ungavela uma izinga le-antigen yegciwane kusampula lingaphansi komkhawulo wokutholwa wokuhlolwa noma uma isampula liqoqwe noma lahanjiswa ngendlela engafanele;ngakho-ke, umphumela wokuhlolwa ongemuhle awukuqedi amathuba okutheleleka nge-COVID-19.
6.Inani le-antigen kusampula lingase lehle njengoba isikhathi sokugula sikhula.Izibonelo eziqoqwe ngemva kosuku lwesi-5 lokugula maningi amathuba okuba zibe negethivu uma kuqhathaniswa nokuhlolwa kwe-RT-PCR.
7.Ukwehluleka ukulandela inqubo yokuhlola kungase kuthinte kabi ukusebenza kokuhlolwa kanye/noma kwenze umphumela wokuhlolwa ungasebenzi.
8.Okuqukethwe kule khithi kuzosetshenziselwa ukutholwa kwekhwalithi yama-antigen e-COVID-19 ezibonelweni zamathe kuphela.
9.I-reagent ingathola kokubili ama-antigen e-COVID-19 asebenzayo futhi angasebenzi. Ukusebenza kokutholwa kuncike ekulayisheni kwe-antigen futhi kungase kungahambelani nezinye izindlela zokuxilonga ezenziwa esibonelweni esifanayo.
10. Imiphumela yokuhlolwa engemihle ayihloselwe ukuthi ilawule ezinye izifo ezingeyona i-COVID-19 ngamagciwane noma amagciwane.
11. Amanani abikezelayo amahle kanye namabi ancike kakhulu emazingeni okuvama.Imiphumela emihle yokuhlolwa kungenzeka imele imiphumela engelona iqiniso phakathi nenkathi yomsebenzi omncane/ongekho we-COVID-19 lapho ukutholakala kwezifo kuphansi.Imiphumela yokuhlolwa engemihle engelona iqiniso ingenzeka kakhulu lapho ukusabalala kwezifo ezibangelwa i-COVID-19 kuphezulu.
12. Lolu cingo luhlolelwe ukusetshenziswa nezinto zesifanekiso somuntu kuphela.
13. Amasosha omzimba e-Monoclonal angase ehluleke ukuthola, noma ukuthola ngokuzwela okuncane, amagciwane e-COVID-19 athole izinguquko ezincane ze-amino acid endaweni ye-epitope eqondiwe.
14. Ukusebenza kwalokhu kuhlolwa akuzange kuhlolwe ukuthi kusetshenziswe ezigulini ezingenazo izimpawu nezimpawu zokutheleleka kokuphefumula kanye nokusebenza kungase kuhluke kubantu abangenazimpawu.
15. Ikhithi yaqinisekiswa ngama-swabs ahlukahlukene.Ukusetshenziswa kwamanye ama-swabs kungase kubangele imiphumela engemihle engamanga.
16. Abasebenzisi kufanele bahlole izifanekiso ngokushesha ngangokunokwenzeka ngemva kokuqoqwa kwesifanekiso.
17. Ukufaneleka kokuhlolwa kwe-Rapid COVID-19 Antigen Test akufakazelwanga ukuhlonza/ukuqinisekisa kokuhlukaniswa kwezicubu zomzimba futhi akufanele kusetshenziswe kulesi sikhundla.