| 64T | 96T | ||
| Isakhi | Umthamo | Isakhi | Umthamo |
| Ipuleti le-reagent | 4 | I-Lysis buffer B | 2 |
| I-Lysis buffer B | 1 | I-Lysis plate | 1 |
| Umkhono wepulasitiki | 8 | Geza ipuleti elingu-1 | 1 |
| Imanuwali yephrothokholi | 1 | Geza amapuleti angu-2 | 1 |
|
|
| Elution plate | 1 |
|
|
| Umkhono wepulasitiki | 1 |
|
|
| Imanuwali yephrothokholi | 1 |
96-well Round Hole Plate Ukulungiswa
Izingxenye ze-64T zepuleti lomthombo elihambisanayo kanje:
| Indawo Kahle | 10r7 | 2or8 | 3 noma 9 | 4or10 | 5orll | 6 nxa2 |
| Ikhithi Isakhi | Lysis Ibhafa 600μL | Geza Isilondolozi1 500μL | Geza I-Buffer2 500μL | Akunalutho | Uzibuthe Ubuhlalu 310μL | Elute Ibhafa l00μL |
Fnoma ikhithi ye-64T:
Susa ngokucophelela ifilimu evala ukushisa epuleti le-reagent, bese wengeza u-200μL wesampula no-20μL we-lysis buffer B kukholamu engu-1/7 yepuleti le-reagent.
Ngekhithi ye-96T:
Susa ngokucophelela ifilimu evala ukushisa epuleti le-reagent, bese wengeza u-200μL wesampula kanye no-20μL we-lysis buffer B ku-lysis plate.
Faka ipuleti le-reagent kanye nomkhono wepulasitiki endaweni ekhethiwe yethuluzi ngohlelo, bese uchofoza ukuze uqalise uhlelo lokukhipha lwe-"DNA/RNA" kusikhiphi se-nucleic acid.
Ekupheleni kohlelo, khipha umkhono wepulasitiki bese uwulahla.
Khipha ipuleti le-elution, bese i-eluent ikhishwa futhi igcinwe kushubhu entsha ye-centrifuge ukuze kuhlolwe umfula.Uma ukuhlola okwehla nomfula kungakwazi ukwenziwa ngesikhathi, isampula ye-DNA ingagcinwa kokuthi -20℃futhi isampula ye-RNA ingagcinwa kokuthi -80℃.