I-SARS-CoV-2 (COVID19) idlangile emhlabeni wonke, futhi ukugoma kwamukelwa njengendlela eyonga kakhulu nephumelelayo yokulawula ubhubhane lwegciwane.Ukuhlolwa kokugoma okujwayelekile ngokuvamile kusebenzisa izindlela zokuhlonza amasosha omzimba ukuze kuhlolwe ukusebenza kahle kwemithi yokugoma ngokuhlolwa kokungathathi hlangothi;
Izindlela zendabuko zidla isikhathi futhi zisebenza kahle kancane, ngokuvamile zithatha izinsuku ezi-2 kuya kwezi-4 ukuqedela ukuhlaziya, futhi ngenxa yokuthi iningi lazo zisebenzisa amagciwane aphilayo, Kudingeka kwenziwe elabhorethri yezinga lesi-3 noma ngaphezulu, okuyisikhathi- kuyadla futhi kuyakhandla, futhi kuletha ukuphazamiseka okukhulu ekuhlaziyeni ukunwetshwa nokuhlanganisa.Ngakho-ke, kunesidingo esiphuthumayo sendlela ehlukile elula nesheshayo efanele ukuhlolwa kwamasosha omzimba avikelayo kubantu abaningi.
I-Aehealth COVID19 Neutralization Antibody Quantitative Test Kit isetshenziselwa ukutholwa kobuningi kwamasosha omzimba anciphisa amandla e-COVID19 ku-serum yomuntu, i-plasma noma igazi lonke.Ingasetshenziselwa ukutholwa okusheshayo, ubuningi nokuzwela kakhulu ku-vitro.esetshenziswa emtholampilo ekuhloleni okusizayo komphumela womuthi omusha wokugomela i-coronavirus kanye nokuhlolwa kwamasosha omzimba anciphisa amandla ezigulini ezilulame ngemva kokutheleleka.
Amasosha omzimba angathathi hlangothi amisa ukutheleleka ngempumelelo ngokuvimbela ukuxhumana phakathi kwegciwane le-COVID19 namaseli aphethe.Iningi lamasosha omzimba angathathi hlangothi liphendula esizindeni sokubopha i-receptor (RBD) sephrotheni ye-spike, ebophezela ngqo ku-cell surface receptor ACE2.ama-antibodies-online okwamanje ahlinzeka ngamasosha omzimba amabili anciphisa ngokususelwe ku-clone CR3022.Ngenkathi amasosha omzimba abopha i-S-protein RBD eqhudelana ngokubopha ama-antigen ne-ACE2, i-epitope ye-CR3022 ayidluleli nesayithi elibopha i-ACE2.
Ngakho-ke akuvimbeli ukubopha amasosha omzimba angathathi hlangothi.Ngenkathi i-CR3022 ngokwayo ikhombisa umphumela obuthakathaka obungangeni, kukhonjisiwe ukuthi isebenzisana namanye amasosha omzimba abopha i-S-protein RBD ukuze enze i-COVID19 ingabi namandla.
Ukusebenza okulula
- Asikho isidingo sokuba ochwepheshe baqeqeshwe;
- Isidingo sesampula esiphansi, sidinga kuphela i-50 μL;
- Ihambisana nohlobo lwezibonelo eziningi: I-Serum/Plasma/Igazi Eliphelele.
Ukuzwela okuphezulu
- Ukuzwela: 98.95%;
- Ukucaciswa: 100%.
Isebenza kahle
- Isikhathi sokusabela: 15 amaminithi, Isikhathi sokuhlola: 10s;
- Protable, Wide ukusetshenziswa izimo;
- Ibhethri elakhelwe ngaphakathi, ukuhlola okungaphezu kuka-200 ngaphandle kokufaka amandla.
Uthembekile
- Kuqinisekiswe izivivinyo zasemtholampilo ezingama-3600, izivivinyo eziyi-1500 yisifanekiso somuntu othelelekile, izivivinyo ezingama-900 kubagomi, izivivinyo eziyi-1200 ngabantu abajwayelekile.;
- Idatha yomtholampilo itholwa kubagomi ngomuthi wokugoma ongacushiwe, umuthi wokugomela i-nucleic acid, umuthi wokugomela amaprotheni, nabantu abangenwe yigciwane, abantu abajwayelekile.
- Nciphisa inani lokuvimbela inani elingu-30%.
Umgomo oyisisekelo osetshenziswa kule kit ukuncintisana kwe-immunochromatographic.Ulayini wokuthola (T-line) emgqeni wokuhlola umbozwe nge-angiotensin converting enzyme 2 futhi ulayini wokulawula (C-line) umbozwe nge-anti-rabbit IgG antibody. I-conjugate pad ihlanganiswe ne-fluorescently ebhalwe ukuthi i-COVID19 RBD protein kanye ne-fluorescently ebhalwe ukuthi unogwaja we-IgG antibody.Ngesikhathi sokutholwa, lapho isampula iqukethe i-antibody okufanele ihlolwe, i-conjugate yento yokuhlola kusampula kanye ne-fluorescent antigen yakha i-immune complex futhi i-immune complex ayikwazi ukubopha i-enzyme 2 eguqula i-angiotensin enganyakazi kulwelwesi lwe-nitrocellulose. .I-fluorescent antigen conjugate engaboshelwe ku-antibody ezohlolwa izobophezela ku-angiotensin converting enzyme 2 enganyakazi kulwelwesi lwe-nitrocellulose ukuze yakhe umugqa wokuthola (T).
- Ukuhlolwa kokuhlolwa ngaphambi kokugoma;
- Ukuqapha imiphumela ngemuva kokugoma;
- Ukuhlolwa kwengozi yokutheleleka kwesibili kwabantu abanaleli gciwane;
- Ukuhlolwa kobungozi kubantu abajwayelekile (okuhlanganisa nokutheleleka ngezimpawu ezingabonakali) ukutheleleka;
- Ukuhlolwa kwekhono lokumelana negciwane.