Le kit isetshenziselwa ukutholwa kwe-in vitro qualitative nucleic acidI-Monkeypox Virus ku-Human serum, amasampula e-lesion exudate kanyeizibonelo zoqweqwe.Amasethi e-Primer kanye nama-FAM anelebula ama-probe aklanyelweukuze kutholwe ngokuqondile i-Monkeypox Virus, isakhi sofuzo se-Human RNase Pekhishwe kanyekanye nesampula yokuhlola inikeza okwangaphakathiukulawula ukuqinisekisa inqubo yokukhipha i-nucleic kanye ne-reagentubuqotho.Uphenyo oluqondiswe kufuzo lomuntu lwe-RNase P lubhalwe ukuthi VIC.
| Izingxenye | 48 Izivivinyo/ikhithi | 96 Izivivinyo/ikhithi |
| Ibhafa yokusabela ye-PCR | 672 μL × 1 ishubhu | 672 μL × 2 ithubhu |
| Ingxube ye-enzyme ye-PCR | 50 μL × 1 ishubhu | 100 μL × 1 ishubhu |
| Ukulawula okuhle | 100 μL × 1 ishubhu | 200 μL × 1 ishubhu |
| Ukulawula okungalungile | 100 μL × 1 ishubhu | 200 μL × 1 ishubhu |
1. Ukuzwela: amakhophi angu-200/mL.
2. Ukucaciswa: Akukho ukusabela okuphambene ne-Enterovirus(EV), Isimungumungwanevirus(MV), Rubella virus(RV),Varicella-zoster virus(VZV),Igciwane lodenga (DenV), HumanI-Parvovirus B19(HPVB19),I-Epstein-barr virus (EBv), i-human herpes virus 6(HHV-6)
3. Ukunemba: I-CV ≤ 5%.
Isistimu ye-PCR Yesikhathi Sangempela: Diagenex AL, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P/3005P, Rotor-GeneTM 6000/Q, Bio-Rad CFX96 TouchTM/iQ ，Hongshi SLAN-96S/96P,AGS8830, AGS4800